Joe Thakuria

Please see below...do you want to send him the 4 other samples?

Lesley, thanks for the email.

Jeffrey, can add whoever he want into the YPO exome study but I will need to have those samples mailed to me directly and received by next Monday at the latest to be included in this sequencing run.

The exomes (at cost) are $1000 each. I'll add this to his invoice which I'll send before the end of the week.

I don't want this getting lost or held up in HMS or MGH interoffice mail. So, please mail to my home address:

Joe Thakuria

He should have (if I remember correctly) 4 extra kits. If he needs more, let me know right away because we'll be cutting it close in terms of getting into this round (with mailing it out to him and then back to me).

The instructions in the pack are straightforward and he just did it recently but, to summarize:

1. do not eat/drink/smoke or chew gum for 30 minutes prior to collecting (eg, if you ate a chili dog, we could get contamination from pork, beef, chicken, tomato, kidney bean, and chili pepper genomes; you'd be surprised how much similarity there is on the DNA level between very different species)

2. spit into the funnel and fill to the indicated line (not all the way to the top so there's room for #3) and make sure this isn't filled with bubbles. If there are bubbles it may need to settle a little so the process typically takes 2 - 5 minutes.

3. There are 2 caps. When done spitting, close the first cap (attached to the funnel the person spits into) so that you hear a click and the liquid in that first cap goes into the tube. That liquid needs to mix with the collected saliva.

4. Then take that first cap off and put the second smaller cap on and close tightly. Make sure the cap is on well and not leaking (or the sample can't be used).

The following are VERY IMPORTANT:

5. You or someone else will need to send me the names and contact info (especially email and phone) for people who are submitting samples.

6. But do not put those names on the tubes or fill out paperwork that came with the tubes. I'll take care of that.

7. You do need to label the tubes though. For the additional 4 samples, you can use the following designations:

YPO 76, YPO 77, YPO 78, YPO 79

8. Obviously I will need to know who is who. (So you'll need to email me: "YPO 76 is Jane doe, YPO 77 is john doe, etc.)

I can't overemphasize the importance of the labeling. If I'm given the YPO number, names, and/or samples are incorrectly matched, the people will get results that are not theirs.

It's actually very straightforward but I want to avoid any mistakes by spelling everything out.

You can mail the tubes back in the boxes (and unfilled paperwork) they came in. But, of course, make sure each tube is labeled with the appropriate YPO # so we know who is who.

Email me with any questions.

Thanks!

Joe

On Mon, Jun 23, 2014 at 11:29 AM, Lesley Groff <████████████████████> wrote:

HI Joe...I see you wanted to provide Jeffrey with some instruction on the kits today...Let me know! thanks, Lesley